Human Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) ELISA Kit
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Description
Human Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Autophagy/Beclin 1 Regulator 1 (AMBRA1) concentrations in tissue homogenates, cell lysates and other biological fluids.
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Product specifications
| Category | ELISA Kits |
| Immunogen Target | Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) |
| Reactivity | Human |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Assay Type | Sandwich |
| Test Range | 0.313 ng/ml - 20 ng/ml |
| Sensitivity | < 0.19 ng/ml |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Size 2 | 5 × 96 tests |
| Size 3 | 10 × 96 tests |
| Form | Lyophilized |
| Tested Applications | ELISA |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Availability | Shipped within 5-12 working days. The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q9C0C7 |
| Gene ID | 55626 |
| OMIM | 611359 |
| Alias | AMBRA1,DCAF3,DDB1 and CUL4-associated factor 3 |
| Background | Elisa kits for AMBRA1 |
| Status | RUO |
| Note | Validity: The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
AMBRA1 or Activating Molecule in BECN1-regulated Autophagy Protein 1, is a key regulator of the autophagy process in mammalian cells. Autophagy is a cellular process involved in the degradation and recycling of damaged organelles, misfolded proteins, and other cellular components to maintain cellular homeostasis and respond to stress conditions. AMBRA1 was originally identified as a positive regulator of autophagy that interacts with Beclin 1 (encoded by the BECN1 gene), a core component of the class III phosphatidylinositol 3-kinase (PI3K) complex involved in autophagosome formation. Through its interactions with Beclin 1 and other autophagy-related proteins, AMBRA1 plays a crucial role in initiating and regulating the autophagy process.AMBRA1 interacts with Beclin 1 and helps to stabilize the Beclin 1 complex, promoting the nucleation of autophagosomal membranes and the initiation of autophagy. AMBRA1 regulates the size and maturation of autophagosomes, the double-membrane vesicles that sequester cargo for degradation during autophagy. It interacts with various proteins involved in autophagosome formation and fusion with lysosomes. AMBRA1 is also involved in crosstalk with signaling pathways that regulate autophagy, such as the mammalian target of rapamycin (mTOR) pathway and the p53 pathway. It can be phosphorylated and regulated by these pathways to modulate autophagy in response to cellular stress and nutrient availability.
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Human AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1) ELISA Kit
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AMBRA1 antibody
WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1, 298 amino acid protein that contains three WD repeats. Localized to cytoplasmic vesicles, AMBRA1 functions to control protein turnover, cell proliferation and cell survival during neuronal development, thereby playing an important role in autophagy and the development of the nervous system. Multiple isoforms of AMBRA1 exist due to alternative spicing events.
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