Human Aconitase 1 (ACO1) Protein
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Description
Recombinant Aconitase 1 (ACO1) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Aconitase 1 (ACO1) |
| Host | E. coli |
| Origin | Human |
| Conjugation | Unconjugated |
| Observed MW | Molecular Weight: Calculated MW: 15.2 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Asp458-Leu586 Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-12 working days. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P21399 |
| Alias | IRP1,ACONS,HEL60,IREB1,IREBP,IREBP1 |
| Background | Protein ACO1 |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
Aconitase 1 (ACO1) is a dual-function iron-sulfur protein located in the cytoplasm, acting as both an enzyme and an iron-responsive element-binding protein (IRP1). As an enzyme, ACO1 catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid (TCA) cycle, contributing to cellular energy production. Under low iron conditions, ACO1 switches to its role as IRP1, binding to iron-responsive elements (IREs) in mRNAs to regulate the expression of genes involved in iron metabolism, such as ferritin and transferrin receptor. This functional versatility allows ACO1 to coordinate cellular energy metabolism and iron homeostasis. Dysregulation of ACO1 is implicated in disorders related to iron overload or deficiency, as well as oxidative stress-induced damage in neurodegenerative diseases.
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The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
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