Human 26S Proteasome Regulatory Subunit 6A (PSMC3) Protein
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Pida mas información
935106861
info@markelab.com
Precio
221€ (10 µg)
Protein PSMC3
proveedor
Abbexareference
abx068718Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant 26S Proteasome Regulatory Subunit 6A (PSMC3) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
26S Proteasome Regulatory Subunit 6A (PSMC3)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 52.7 kDa Observed MW (SDS-PAGE): 53 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Asn2-Ala439 Tag: N-terminal His tag
expression
Recombinant
purity
> 97%
buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
PSMC3
dry ice
No
availability
Shipped within 5-7 working days.
uniprot id
NCBI accession
NM_002804
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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