Human 26S Proteasome Regulatory Subunit 6A (PSMC3) Protein

Human 26S Proteasome Regulatory Subunit 6A (PSMC3) Protein

Pida mas información

935106861

info@markelab.com

Precio

221€ (10 µg)

Protein PSMC3

proveedor

Abbexa

reference

abx068718

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Recombinant 26S Proteasome Regulatory Subunit 6A (PSMC3) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

26S Proteasome Regulatory Subunit 6A (PSMC3)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 52.7 kDa Observed MW (SDS-PAGE): 53 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Asn2-Ala439 Tag: N-terminal His tag


expression

Recombinant


purity

> 97%


buffer

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

PSMC3


dry ice

No


availability

Shipped within 5-7 working days.


NCBI accession

NM_002804


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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