Heat Shock 70kDa Protein 1A (HSPA1A) Antibody Pair

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1482€ (5 × 96 tests)

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935106861
info@markelab.com
name
Heat Shock 70kDa Protein 1A (HSPA1A) Antibody Pair
category
Antibody Pairs
provider
Abbexa
reference
abx370111
tested applications
ELISA

Description

Heat Shock 70kDa Protein 1A (HSPA1A) Antibody Pair for use in Sandwich ELISA assay development.
This antibody pair contains:
Detection: 50 µg
Capture: 200 µg
Standard: 2 µg

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Antibody Pairs
Immunogen Target
Heat Shock 70kDa Protein 1A (HSPA1A)
Reactivity
Pig
Assay Data
Sandwich
Assay Type
Capture Antibody: 0.5 mg/ml  Biotin-Conjugated Detection Antibody: 0.2 mg/ml
Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user.
Recommended Dilution
Dilute the Capture Antibody 125-fold with Coating Buffer.  Dilute the Biotin-Conjugated Detection Antibody 200-fold with Detection Antibody Diluent.  Optimal dilutions/concentrations should be determined by the end user.
Conjugation
Biotin
Size 1
5 × 96 tests
Size 2
10 × 96 tests
Form
Standard form: Lyophilized
Capture Ab: Liquid
Detection Ab: Liquid
Tested Applications
ELISA
Buffer
The Capture and Detection Antibody both contain 0.1% sodium azide.
Availability
Please enquire.
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Dry Ice
No
Background
Antibody pair for HSPA1A
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Descripción

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HSPA1A, collectively known as HSP70(also referred to HSP72), is a stress-inducible member of heat-shock protein 70(HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines.

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HSPA1A, collectively known as HSP70(also referred to HSP72), is a stress-inducible member of heat-shock protein 70(HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines.(21373891)

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