Glucokinase (GCK) Assay Kit

Description

Glucokinase (GCK) Assay Kit Assay Kit is an assay kit for detection and quantification of Glucokinase concentrations in tissue homogenates, cell lysates and cell culture supernatants using a microplate reader. Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet beta-cells, and has a stronger affinity for glucose than other hexokinases. GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The absorbance values of the standard tube and the sample tube are measured respectively, and the Glucokinase content in the sample can be calculated.