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Dopamine Research ELISA

Research enzyme immunoassay (ELISA) for the quantitative and ultra sensitive determination of dopamine in different species and various biological samples.

Proveedor

LDN

Reference

BAE5300R

Tested Applications

Elisa

Reactivity

Human

Status

RUO

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
96 test
storage
2 – 8 °C
test_range
0 / 0.5 – 80 ng/ml
sensitivity
3.3 pg/ml (depending on sample volume)
assay_data
4 hours, overnight
note
1 – 750 µl, various biological fluids
reserach_area
Catecholamines Research
background
Enzyme Immunoassay for the quantitative determination of dopamine. Flexible test system for various biological sample types and volumes. Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.