DNA Polymerase High Fidelity (HiFi) Enzyme
Por favor contáctenos para obtener información detallada sobre el precio y disponibilidad.
Description
DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T-DNA Polymerase and a proofreading 3'-5' exonuclease. This kit contains two different buffers: HiFi Buffer 1 is optimized for the amplification of genomic DNA, and HiFi Buffer 2 is optimized for the amplification of λDNA, cDNA or plasmid DNA. The extension rate is about 1-2 kb/min. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.
Documents del producto
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | DNA Polymerase (HiFi) |
| Conjugation | Unconjugated |
| Observed MW | Concentration: 5 U/µl |
| Purity | > 99% (SDS-PAGE) |
| Size 1 | 250 U |
| Size 2 | 500 U |
| Size 3 | 3 kU |
| Tested Applications | PCR |
| Buffer | HiFi DNA Polymerase: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. <br> 10X HiFi Buffer 1/2: 200 mM Tris-HCl (pH 9.0), 100 mM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, 100 mM KCl, 10% glycerol, other proprietary ingredients. |
| Availability | Shipped within 10-20 working days. |
| Storage | Store at -20 °C for up to 2 years. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Background | Protein DNA POLY |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
Related Products
DNA Polymerase (6 kb/min) Enzyme
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. The extension rate is about 6 kb/min.
Ver ProductoDNA Polymerase (6 kb/min) Enzyme (with 2.5 mM dNTPs)
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. Amplification of genomic DNA fragment up to 4 kb.
Ver ProductoDNA Polymerase (1-2 kb/min) Enzyme
DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. The extension rate is about 1-2 kb/min. The enzyme can amplify genomic DNA fragments up to 4 kb. This kit does not contain 2.5 mM dNTPs.
Ver Producto