DNA Polymerase for PAGE Enzyme (with 2.5 mM dNTPs)
338€ (2500 U)
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Name
DNA Polymerase for PAGE Enzyme (with 2.5 mM dNTPs)
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx071007
Tested Applications
PCR
Description
DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. The extension rate is about 1-2 kb/min. This enzyme is supplied with a unique buffer compatible with PAGE, and the resulting PCR product is suitable for use in SDS-PAGE and agarose gel electrophoresis. The enzyme can amplify genomic DNA fragments up to 3 kb.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Proteins and Peptides |
| Immunogen Target | DNA Polymerase |
| Host | Bacteria |
| Assay Type | Biological Activity: One unit of DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74 °C. |
| Conjugation | Unconjugated |
| Purity | > 99% (SDS-PAGE) |
| Size 1 | 2500 U |
| Size 2 | 10 kU |
| Tested Applications | PCR |
| Buffer | DNA Polymerase for PAGE: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. <br> 10X Buffer for PAGE: Contains 200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4. |
| Availability | Shipped within 10-20 working days. |
| Storage | Store at -20 °C for up to 2 years. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Background | Protein DNA POLY |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. Endotoxin Level: Functional absence of double and single stranded endonuclease activity. Concentration: 5 U/µl |
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