DNA Polymerase for PAGE Enzyme
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Description
DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. The extension rate is about 1-2 kb/min. This enzyme is supplied with a unique buffer compatible with PAGE, and the resulting PCR product is suitable for use in SDS-PAGE and agarose gel electrophoresis. The enzyme can amplify genomic DNA fragments up to 3 kb. This kit does not contain 2.5 mM dNTPs.
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | DNA Polymerase |
| Host | Bacteria |
| Conjugation | Unconjugated |
| Observed MW | Concentration: 5 U/µl |
| Purity | > 99% (SDS-PAGE) |
| Size 1 | 2500 U |
| Size 2 | 10 kU |
| Tested Applications | PCR |
| Buffer | DNA Polymerase for PAGE: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. <br> 10X Buffer for PAGE: Contains 200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4. |
| Availability | Shipped within 10-20 working days. |
| Storage | Store at -20 °C for up to 2 years. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Background | Protein DNA POLY |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
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