Calcein AM/PI Mammalian Cell Viability Assay Kit

Description

Calcein AM/PI Cell Viability Assay Kit is an assay kit for the diffferentiation of living and dead mammalian cells with esterase activity. A cell-pemeable non-fluorescent fluorescein (Calcein AM) is catalysed by cytosolic esterase to produce the cell-impermeable green fluorescent probe Calcein. Dead cells show limited esterase activity, and so do not strongly produce Calcein. Following cell death, selective membrane permeability is lost, and the red fluorescent probe Propidium Iodide can enter cells to bind duplex DNA. Living cells show green fluorescence, and dead cells show red fluorescence.