Bromodeoxyuridine (BrdU) Antibody
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Description
BrdU Antibody is a Mouse Monoclonal against BrdU.
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | Bromodeoxyuridine (BrdU) |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Recommended Dilution | IHC: 1/200 - 1/1000, IF/ICC: 1/50 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Isotype | IgG2a |
| Purity | ≥ 95% (SDS-PAGE) |
| Purification | Purified by Protein A and Protein G affinity chromatography. |
| Size 1 | 100 µg |
| Form | Liquid |
| Tested Applications | ELISA, IHC, IF/ICC |
| Buffer | PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol. |
| Availability | Shipped within 5-12 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Background | Antibody anti-BrdU |
| Status | RUO |
| Note | Concentration: 2 mg/ml - Validity: 12 months. |
Descripción
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BrdU antibody
Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
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Bromodeoxyuridine (BrdU) Antibody
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BrdU (5-bromo-2-deoxyuridine) Antibody is a Mouse Monoclonal against BrdU. This antibody was produced in BALB/c mice. This antibody can be used to detect profilerating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells, in place of thymidine during DNA replication.
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