Beta Actin (ACTB) Protein
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Description
Actin Beta Protein is a recombinant protein expressed in E. coli. Shares sequence homology with: Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Horse, Chicken, Guinea pig.
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Beta Actin (ACTB) |
| Host | E. coli |
| Origin | Human, Mouse, Rat, Guinea pig, Dog, Pig, Cow, Sheep, Horse, Chicken |
| Conjugation | Unconjugated |
| Observed MW | Molecular Weight: Calculated MW: 43.3 kDa Observed MW (SDS-PAGE): 44 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Phe375 Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 90% |
| Size 1 | 50 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 500 µg |
| Size 5 | 1 mg |
| Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P60709, P60710, P60711 |
| Gene ID | 60, 11461, 81822 |
| Alias | BKRNS,BNS,BRWS1,CSMH,DDS1,THC8 |
| Background | Protein ACTB |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
ACTB, also known as beta-actin, is a highly conserved protein that serves as a fundamental component of the cytoskeleton in eukaryotic cells. Beta-actin is involved in various cellular processes, including cell structure, motility, and intracellular transport. Beta-actin forms filamentous structures, along with other actin isoforms, which constitute the cytoskeleton. This dynamic network of filaments provides structural support to the cell and is involved in cell shape determination and mechanical stability, motility and migration. It participates in the formation of cell protrusions such as lamellipodia and filopodia, which are essential for cell movement. Beta-actin also plays a role in cell division, where it is involved in cytokinesis, the process of cell division following nuclear division. Furthermore, beta-actin is involved in intracellular transport processes, serving as a track for myosin motor proteins to transport cargo within the cell. This function is essential for the movement of vesicles, organelles, and other cellular components along the cytoskeleton.
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Beta Actin (ACTB) Antibody
Beta actin Antibody is a Mouse Monoclonal antibody against Bet actin. Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.
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