HIF1A antibody
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Description
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) (HIF1A) |
| Host | Mouse |
| Reactivity | Human |
| Recommended Dilution | WB: 1:1000-1:10000; IF: 1:50-1:200 |
| Clonality | monoclonal |
| Conjugation | Unconjugated |
| Isotype | IgG1 |
| Clone ID | 5H12 |
| Observed MW | 120 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | protein A+G purified |
| Size 1 | 100µg |
| Form | liquid |
| Tested Applications | ELISA, IF, WB |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| UniProt ID | Q16665 |
| Gene ID | 3091 |
| Alias | Hypoxia-inducible factor 1-alpha (HIF-1-alpha, HIF1-alpha),ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78 (bHLHe78),Member of PAS protein 1,PAS domain-containing protein 8,HIF1A,BHLHE78,MOP1,PASD8 |
| Background | Antibody anti-HIF1A |
| Status | RUO |
| Note | Mol. Weight 120 kDa |
Descripción
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HIF1A antibody
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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