Actin Beta (ACTb) Antibody Pair
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Description
Actin Beta (ACTb) Antibody Pair for use in Sandwich ELISA assay development.
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Product specifications
| Category | Antibody Pairs |
| Immunogen Target | Actin Beta (ACTb) |
| Reactivity | Human, Mouse, Rat |
| Assay Data | Detection Antibody Biotinilated |
| Assay Type | Sandwich |
| Recommended Dilution | Dilute the Capture Antibody 125-fold with Coating Buffer. Dilute the Biotin-Conjugated Detection Antibody 200-fold with Detection Antibody Diluent. Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 5 × 96 tests |
| Size 2 | 10 × 96 tests |
| Form | Standard: Lyophilized--Liquid (Capture Antibody and Detection Antibody) Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. |
| Tested Applications | ELISA |
| Buffer | The Capture and Detection Antibody both contain 0.1% sodium azide. |
| Availability | Please enquire. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Alias | BKRNS,BNS,BRWS1,CSMH,DDS1,THC8 |
| Background | Antibody Pair for ACTB |
| Status | RUO |
| Note | This antibody pair contains ( 5 x 96 det): Detection: 50 µg Capture: 200 µg Standard: 2 µg This product is for research use only. |
Descripción
ACTB, also known as beta-actin, is a highly conserved protein that serves as a fundamental component of the cytoskeleton in eukaryotic cells. Beta-actin is involved in various cellular processes, including cell structure, motility, and intracellular transport. Beta-actin forms filamentous structures, along with other actin isoforms, which constitute the cytoskeleton. This dynamic network of filaments provides structural support to the cell and is involved in cell shape determination and mechanical stability, motility and migration. It participates in the formation of cell protrusions such as lamellipodia and filopodia, which are essential for cell movement. Beta-actin also plays a role in cell division, where it is involved in cytokinesis, the process of cell division following nuclear division. Furthermore, beta-actin is involved in intracellular transport processes, serving as a track for myosin motor proteins to transport cargo within the cell. This function is essential for the movement of vesicles, organelles, and other cellular components along the cytoskeleton.
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Beta Actin (ACTB) Antibody
Beta actin Antibody is a Mouse Monoclonal antibody against Bet actin. Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.
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