3-CAT ELISA Fast Track

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935106861

info@markelab.com

Precio

Precio a consultar

proveedor

LDN

reference

BA E-6600

Tested Applications

Elisa

reactivity

human

status

CE Marked

clonality

Descripción

Fast Track enzyme immunoassay (ELISA) for the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine.


Fast Track enzyme immunoassay (ELISA) para la determinación cuantitativa de adrenalina (epinefrina), noradrenalina (norepinefrina) y dopamina en plasma y orina.

Background

Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine. Adrenaline (epinephrine), noradrenaline (norepinephrine), and dopamine are extracted by using a cis-diolspecific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm


Inmunoensayo enzimático para la determinación cuantitativa de adrenalina (epinefrina), noradrenalina (norepinefrina) y dopamina en plasma y orina. La adrenalina (epinefrina), noradrenalina (norepinefrina) y dopamina se extraen utilizando un gel de afinidad específica para cis-dioles, se acilan y luego se convierten enzimáticamente. El kit ELISA competitivo utiliza el formato de microplaca. El antígeno se une a la fase sólida de la microplaca. Los estándares derivatizados, los controles y las muestras, así como los analitos unidos a la fase sólida, compiten por un número fijo de sitios de unión de anticuerpos. Después de que el sistema está en equilibrio, se eliminan el antígeno libre y los complejos antígeno-anticuerpo libre mediante lavado. El anticuerpo unido a la fase sólida se detecta mediante un conjugado de anti-IgG de conejo peroxidasa usando TMB como sustrato. La reacción se lee a 450 nm.

Características del producto

category

ELISA Kits


size 1

3 x 96 det


storage

2 – 8 °C


test range

Adrenaline: 0 / 1 – 200 ng/mlNoradrenaline: 0 / 5 – 1 000 ng/mlDopamine: 0 / 4.5 – 2 000 ng/ml


detection method

Extraction and acylation in 48-well microtiter plates. 6 standards. 2 controls. Ready for use.


assay data

4 hours 40 min


availability

Inmediate


dry ice

No


note

300 µl plasma; 10 µl urine


alias

Adrenaline, Noradrenaline,Dopamine,Adrenalina, Noradrenalina,Dopamina,Epinephrine,Epinefrina,Norepinefrina,Norepinephrine


reseach area

Catecholamines


status

CE Marked


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