Características de producto
2 – 8 °C
0 / 2.5 – 250 µg/ml
Common sample preparation for all biological fluids. Derivatization in liquid phase. 96-well-reaction plate. 12 x 8 break apart wells microtiter plate. 6 standards. 2 controls. Ready for use.
Sample preparation 2 hours 30 min; ELISA overnight, 2 x 30 min
20 µl urine, plasma or serum
Enzyme Immunoassay for the quantitative determination of Tryptophan in urine, serum and plasma samples. After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.