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Serotonin Research ELISA

Research enzyme immunoassay (ELISA) for the quantitative ultra sensitive determination of serotonin in various biological samples.

Proveedor

LDN

Reference

BAE5900R

Tested Applications

Elisa

Reactivity

Human

Status

RUO

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
96 test
storage
2 – 8 °C
test_range
0 / 0.015 – 2.5 ng/ml
sensitivity
5 pg/ml (depending on sample volume)
detection_method
Common sample preparation for various biological fluids. Acylation in 96-well-acylation plate. 12 x 8 break apart wells microtiter plate. 6 standards. 2 controls. Ready for use. Ultra-sensitive ELISA.
assay_data
Acylation 30 min; ELISA overnight, 2 x 30 min
availability
Inmediate
dry_ice
No
note
1 – 100 µl, various biological fluids
reserach_area
Serotonin
background
Ultra-sensitive Enzyme Immunoassay for the quantitative determination of Serotonin. Flexible test system for various biological sample types and volumes. Serotonin is acylated and detected by the subsequent competitive ELISA kit, which uses the microtiter plate format. Antigens are bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm