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Serotonin ELISA Fast Track
Características de producto
2 – 8 °C
0 / 15 – 2 500 ng/ml
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
Acylation 15 min; ELISA 1 hour
25 µl serum, urine or platelets
Enzyme Immunoassay for the quantitative determination of Serotonin in serum, urine and platelets. In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm