proveedor
FineTestreference
P2456Tested Applications
Western Blot, ELISAreactivity
status
RUOclonality
Background
MMPs are secreted by diverse connective tissue and pro-inflammatory cells. Typically, these enzymes exhibit minimal expression under normal physiological circumstances. MMPs assume a pivotal role in various cellular processes, including cell proliferation, migration, differentiation, angiogenesis, apoptosis, and immunity. They share a common domain structure, consisting of three key components. First is the pro-peptide, which requires removal to activate the enzyme. Next, there is the catalytic domain, featuring a cysteine switch, where a cysteine residue interacts with zinc, and the haemopexin-like C-terminal domain is linked to the catalytic domain via a flexible hinge region. TIMP-3 (Tissue Inhibitor of Metalloproteinases 3) serves as a natural inhibitor of matrix metalloproteinases (MMPs). It has the ability to form complexes with various metalloproteinases, including collagenases, and inactivates them irreversibly by binding to their catalytic zinc cofactor. This binding effectively halts the enzymatic activity of these MMPs. TIMP-3 is known to act on several MMPs, including MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14, and MMP-15. Its role in forming tissue-specific acute responses to remodeling stimuli underscores its importance in regulating extracellular matrix dynamics and tissue maintenance.
MMPs son secretadas por diversos tejidos conectivos y células proinflamatorias. Normalmente, estas enzimas exhiben una expresión mínima en circunstancias fisiológicas normales. Las MMP asumen un papel fundamental en diversos procesos celulares, incluida la proliferación, migración, diferenciación, angiogénesis, apoptosis e inmunidad celular. Comparten una estructura de dominio común, que consta de tres componentes clave. El primero es el propéptido, que requiere eliminación para activar la enzima. A continuación, está el dominio catalítico, que presenta un interruptor de cisteína, donde un residuo de cisteína interactúa con el zinc, y el dominio C-terminal similar a hemopexina está unido al dominio catalítico a través de una región bisagra flexible. TIMP-3 (inhibidor tisular de metaloproteinasas 3) sirve como inhibidor natural de las metaloproteinasas de matriz (MMP). Tiene la capacidad de formar complejos con varias metaloproteinasas, incluidas las colagenasas, y las inactiva irreversiblemente uniéndose a su cofactor catalítico de zinc. Esta unión detiene efectivamente la actividad enzimática de estas MMP. Se sabe que TIMP-3 actúa sobre varias MMP, incluidas MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 y MMP-15. Su papel en la formación de respuestas agudas específicas de tejido a estímulos de remodelación subraya su importancia en la regulación de la dinámica de la matriz extracelular y el mantenimiento del tejido
Características del producto
alias
HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, Protein MIG 5, SFD, Timp 3, TIMP3
availability
7 days
buffer
Reconstitute with Sterile distilled water
category
Proteins and Peptides
expression
Recombinant
form
Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
host
E.Coli
immunogen target
158-211
note
This product is for research use only.
observerd MW
26.8 kDa
origin
Human
purity
Greater than 50% by SDS-PAGE gel analyses
purification
IF2DI tag
size 1
50μg
size 2
200μg
size 3
1mg
status
RUO
storage
-20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution
uniprot id
P35625
Quizá le pueda interesar
Chicken TIMP-3(Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
proveedor
FineTestreference
ECH0066reactivity
chickenstatus
RUOPrecio a consultar
Ver másHuman TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit
proveedor
FineTestreference
EH0296reactivity
humanstatus
RUOPrecio a consultar
Ver másRat Metalloproteinase Inhibitor 3 (TIMP3) CLIA Kit
proveedor
Abbexareference
abx190717Tested Applications
CLIAreactivity
Ratstatus
RUORat Tissue Inhibitors of Metalloproteinase 3 (TIMP3) Chemiluminescent Immunoassay (CLIA) Kit is a Sandwich Chemiluminesc...
812.5€ (96 tests)
Ver más