proveedor
LDNreference
BA E-8500Tested Applications
Elisareactivity
humanstatus
CE Markedclonality
Descripción
Fast Track enzyme immunoassay (ELISA) for the quantitative determination of normetanephrine in urine.
Fast Track enzyme immunoassay (ELISA) para la determinación cuantitativa de normetanefrina en orina.
Background
Enzyme Immunoassay for the quantitative determination of Normetanephrine in urine. During the sample preparation Normetanephrine (Normetadrenaline) is quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
Ensayo inmunoenzimático para la determinación cuantitativa de Normetanefrina en la orina. Durante la preparación de la muestra, la Normetanefrina(Normetadrenalina) se acila cuantitativamente. A continuación, se utiliza un ELISA competitivo en formato de microplaca. El antígeno se une a la fase sólida de la microplaca. Los estándares acilados, los controles y las muestras, así como el analito unido a la fase sólida, compiten por un número fijo de sitios de unión de anticuerpos. Después de que el sistema esté en equilibrio, el antígeno libre y los complejos antígeno-anticuerpo libres se eliminan mediante lavado. El anticuerpo unido a la fase sólida se detecta mediante un conjugado de anti-IgG-peroxidasa (conejo) utilizando TMB como sustrato. La reacción se lee a 450 nm
Características del producto
alias
Normetanefrine,Normetanefrina
assay data
Hydrolysis and acylation 45 min; ELISA 1 hour
availability
Inmediate
category
ELISA Kits
detection method
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
dry ice
No
note
25 µl urine
reseach area
Metanephrine and Normetanephrine in Urine
size 1
96 test
status
CE Marked
storage
2 – 8 °C
test range
0 / 30 – 3 000 ng/ml
Quizá le pueda interesar
Metanephrine Urine ELISA Fast Track
proveedor
LDNreference
BA E-8400Tested Applications
Elisareactivity
humanstatus
CE MarkedFast Track enzyme immunoassay (ELISA) for the quantitative determination of metanephrine in urine.
Precio a consultar
Ver másNormetanephrine Urine ELISA Fast Track
proveedor
LDNreference
BA E-8500Tested Applications
Elisareactivity
humanstatus
CE MarkedFast Track enzyme immunoassay (ELISA) for the quantitative determination of normetanephrine in urine.
Precio a consultar
Ver más2-MET Urine ELISA Fast Track
proveedor
LDNreference
BA E-8600Tested Applications
Elisareactivity
humanstatus
CE MarkedFast Track enzyme immunoassay (ELISA) for the quantitative determination of metanephrine and normetanephrine in urine.
Precio a consultar
Ver más