Normetanephrine Urine ELISA Fast Track
Características de producto
2 – 8 °C
0 / 30 – 3 000 ng/ml
LOD 14.7 ng/mlLOB 10.4 ng/mlLOQ 16.2 ng/ml
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
Hydrolysis and acylation 45 min; ELISA 1 hour
25 µl urine
Metanephrine and Normetanephrine in Urine
Enzyme Immunoassay for the quantitative determination of Normetanephrine in urine. During the sample preparation Normetanephrine (Normetadrenaline) is quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.