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Histamine ELISA

Enzyme immunoassay (ELISA) for the quantitative determination of histamine in plasma and urine.

Proveedor

LDN

Reference

BAE1000

Tested Applications

Elisa

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
96 test
storage
2 – 8 °C
test_range
0 / 0.5 – 50 ng/ml
sensitivity
0.18 ng/ml plasma; 0.22 ng/ml urine
detection_method
Common sample preparation. Acylation in liquid phase. 96-well acylation plate. 12 x 8 break apart wells microtiter plate. 6 Standards. 2 Controls. Ready for use.
assay_data
4 hours
availability
Inmediate
dry_ice
No
note
25 µl plasma; 10 µl urine
reserach_area
Histamine
background
Enzyme Immunoassay for the quantitative determination of Histamine in plasma and urine. In combination with the supplementary kit Histamine Release (for details contact your local supplier), the assay can be used for the measurement of histamine release in heparinized whole blood. In the first part of the procedure, Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.