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5-HIAA ELISA
Enzyme immunoassay (ELISA) for the quantitative determination of 5-HIAA (5-hydroxyindoleacetic acid) in urine.
Proveedor
LDN
Reference
BAE1900
Tested Applications
Elisa
Reactivity
Human
Status
CE Marked
Download instructionsCaracterísticas de producto
tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
96 test
storage
2 – 8 °C
test_range
0 / 0.5 – 50 mg/l
sensitivity
0.17 mg/l
detection_method
Common sample preparation. 6 standards. 2 controls. Ready for use.
availability
Inmediate
dry_ice
No
note
50 µl urine
reserach_area
5-HIAA
background
Enzyme Immunoassay for the quantitative determination of 5-Hydroxy-3-Indole Acetic Acid (5-HIAA) in urine. First, 5-HIAA is derivatized by methylation. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The methylated analyte in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system has reached equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm