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2-MET Urine RIA Fast Track

Fast Track radioimmunoassay (RIA) for the quantitative determination of metanephrine and normetanephrine in urine.

Proveedor

LDN

Reference

BAR8600

Tested Applications

RIA

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
RIA
reactivity
Human
category
RIA Kits
size_1
2 x 100 det.
storage
2 – 8 °C
test_range
Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/ml
sensitivity
Metanephrine:LOD 9.0 ng/mlLOB 6.2 ng/mlLOQ 10.7 ng/mlLOD 10.4 ng/mlLOB 7.4 ng/mlLOQ 11.4 ng/ml
detection_method
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use. Double antibody technique.
assay_data
Hydrolysis and acylation 45 min; RIA 1 hour 30 min
availability
Inmediate
dry_ice
No
note
25 µl urine
reserach_area
Metanephrine and Normetanephrine in Urine
background
125I – Radioimmunoassay for the quantitative determination of metanephrine and normetanephrine in urine. First, metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125Ilabelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards