2-MET Urine RIA Fast Track
Características de producto
2 x 100 det.
2 – 8 °C
Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/ml
Metanephrine:LOD 9.0 ng/mlLOB 6.2 ng/mlLOQ 10.7 ng/mlLOD 10.4 ng/mlLOB 7.4 ng/mlLOQ 11.4 ng/ml
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use. Double antibody technique.
Hydrolysis and acylation 45 min; RIA 1 hour 30 min
25 µl urine
Metanephrine and Normetanephrine in Urine
125I – Radioimmunoassay for the quantitative determination of metanephrine and normetanephrine in urine. First, metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125Ilabelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards