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2-MET Urine ELISA Fast Track

Fast Track enzyme immunoassay (ELISA) for the quantitative determination of metanephrine and normetanephrine in urine.

Proveedor

LDN

Reference

BAE8600

Tested Applications

Elisa

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
2 x 96 det
storage
2 – 8 °C
test_range
Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/mlSensitivity:
sensitivity
LOD 8.6 ng/mlLOB 6.0 ng/mlLOQ 10.5 ng/ml
detection_method
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
assay_data
Hydrolysis and acylation 45 min; ELISA 1 hour
availability
Inmediate
dry_ice
No
note
25 µl urine
reserach_area
Metanephrine and Normetanephrine in Urine
background
Enzyme Immunoassay for the quantitative determination of Metanephrine and Normetanephrine in urine. During the sample preparation Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm