proveedor
LDNreference
BA E-8600Tested Applications
Elisareactivity
humanstatus
CE Markedclonality
Descripción
Fast Track enzyme immunoassay (ELISA) for the quantitative determination of metanephrine and normetanephrine in urine.
Fast Track enzyme immunoassay (ELISA) para la determinación cuantitativa de metanefrina y normetanefrina en orina.
Background
Enzyme Immunoassay for the quantitative determination of Metanephrine and Normetanephrine in urine. During the sample preparation Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm
Inmunoensayo enzimático para la determinación cuantitativa de Metanefrina y Normetanefrina en la orina. Durante la preparación de la muestra, la Metanefrina (Metadrenalina) y la Normetanefrina (Normetadrenalina) se acilan cuantitativamente. El kit de ELISA competitivo posterior utiliza el formato de microplaca. El antígeno se une a la fase sólida de la microplaca. Los estándares acilados, los controles y las muestras, así como los analitos unidos a la fase sólida, compiten por un número fijo de sitios de unión de anticuerpos. Después de que el sistema esté en equilibrio, el antígeno libre y los complejos antígeno-anticuerpo libres se eliminan mediante lavado. El anticuerpo unido a la fase sólida se detecta mediante un conjugado de anti-IgG-peroxidasa (Cconejo) utilizando TMB como sustrato. La reacción se lee a 450 nm.
Características del producto
alias
Metanephrine, Normetanephrine, Metanefrina,Normetanefrina,Metanefrine
assay data
Hydrolysis and acylation 45 min; ELISA 1 hour
availability
Inmediate
category
ELISA Kits
detection method
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
dry ice
No
note
25 µl urine
reseach area
Metanephrine and Normetanephrine in Urine
size 1
2 x 96 det
status
CE Marked
storage
2 – 8 °C
test range
Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/mlSensitivity:
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