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2-MET Plasma RIA Fast Track

Fast Track radioimmunoassay (RIA) for the quantitative determination of free metanephrine and free normetanephrine in plasma.

Proveedor

LDN

Reference

BAR8300

Tested Applications

RIA

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
RIA
reactivity
Human
category
RIA Kits
size_1
2 x 96 det
storage
2 – 8 °C
test_range
Metanephrine: 0 / 36 – 3 600 pg/mlNormetanephrine 0 / 72 – 7 200 pg/ml
sensitivity
Metanephrine: 5.8 pg/mlNormetanephrine: 21.4 pg/ml
detection_method
Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.
assay_data
Extraction 3 hours; RIA overnight 30 min
availability
Inmediate
dry_ice
No
note
200 µl plasma
reserach_area
Free Plasma Metanephrines
background
Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are first extracted using an ion exchange matrix followed by an acylation process. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125Ilabelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards