2-CAT ELISA Fast Track
Características de producto
2 x 96 det
2 – 8 °C
Adrenaline: 0 / 1 – 200 ng/ml: Noradrenaline: 0/5-1000ng/ml
Adrenaline: 0.01 ng/ml plasma; 0.9 ng/ml urine. Noradrenaline. 0.036ng/ml Plasma;1.7 ng/ml Urine
Extraction and acylation in 48-well microtiter plates. 6 standards. 2 controls. Ready for use.
4 hours 40 min
300 µl plasma; 10 µl urine
Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma and urine. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.