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2-CAT ELISA Fast Track

Fast Track enzyme immunoassay (ELISA) for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma and urine.

Proveedor

LDN

Reference

BAE6500

Tested Applications

Elisa

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
2 x 96 det
storage
2 – 8 °C
test_range
Adrenaline: 0 / 1 – 200 ng/ml: Noradrenaline: 0/5-1000ng/ml
sensitivity
Adrenaline: 0.01 ng/ml plasma; 0.9 ng/ml urine. Noradrenaline. 0.036ng/ml Plasma;1.7 ng/ml Urine
detection_method
Extraction and acylation in 48-well microtiter plates. 6 standards. 2 controls. Ready for use.
assay_data
4 hours 40 min
availability
Inmediate
dry_ice
No
note
300 µl plasma; 10 µl urine
reserach_area
Catecholamines
background
Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma and urine. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.