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2-CAT (A-N) Research ELISA

Research enzyme immunoassay (ELISA) for the quantitative and ultra sensitive determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in different species and various biological samples.

Proveedor

LDN

Reference

BAE5400R

Tested Applications

Elisa

Reactivity

Human

Status

RUO

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
2 x 96 det
storage
2 – 8 °C
test_range
Adrenaline: 0 / 0.5 – 80 ng/mlNoradrenaline: 0 / 0.2 – 32 ng/ml
sensitivity
Adrenaline: 6.6 pg/ml (depending on sample volume)Noradrenaline: 2.6 pg/ml (depending on sample volume)
assay_data
4 hours, overnight
availability
Inmediate
dry_ice
No
note
1 – 750 µl, various biological fluids
reserach_area
Catecholamines Research
background
Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine). Flexible test system for various biological sample types and volumes. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.